Neuropeptide Y facilitates P2X1 receptor-dependent vasoconstriction via Y1 receptor activation in small mesenteric arteries during sympathetic neurogenic responses

ATP, norepinephrine and NPY are co-released by sympathetic nerves innervating arteries. ATP elicits vasoconstriction via activation of smooth muscle P2X receptors. The functional interaction between neuropeptide Y (NPY) receptors in arteries is not known. In this study we investigate the effect on P2X1-dependent mouse mesenteric Suramin or P2X1 antagonist NF449 abolished α,β-meATP evoked vasoconstrictions. lacked any direct vasoconstrictor but facilitated vasoconstrictive response to α,β-meATP. Mesenteric expressed Y1 Y4 receptors, Y2 Y5. receptor inhibition (BIBO3304) reversed facilitation α,β-meATP-evoked vasoconstriction. L-type Ca2+ channel antagonism (nifedipine) had no vasoconstrictions, completely facilitation. Electrical field stimulation neurogenic Neurogenic responses were dependent upon dual α1-adrenergic (prazosin) (NF449) activation. partially reduced Isolation component blockade allowed faciliatory effects be explored. during post-junctional conclusion, have identified that lacks a can facilitate enhancing activity P2X1, following exogenous agonists nerve stimulation. mechanism involved channel.